Fig. 3.

qRT-PCR quantification of mRNA expression of T-cell-related genes in the brain. RNA was extracted from brains of mock- and JEV-infected surviving and dying mice at 13 dpi (n = 5). (A) mRNA expression of CD3, CD4, CD8, CD25, and CD69 as T-cell-related antigens, (B) perforin, Gzm A, and Gzm B as cytotoxic granules, and (C) Treg-cell-related genes Foxp3 and TGFβ1 is shown. The mRNA expression levels in JEV-infected brains were normalized to GAPDH expression. Vertical error bars indicate the standard deviation (SD) of three independent experiments. Significant differences (p < 0.05, ANOVA) between surviving and dying mouse brain at 13 dpi are indicated by an asterisk (*)