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. 2014 Dec 24;14:85. doi: 10.1186/s12935-014-0085-9

Figure 2.

Figure 2

qPCR detection of the peaks of melting curves for each transcript. The peaks of the melting curves of PCR products for T-bet, GATA-3, EGR-1, TGF-β, and GAPDH reference gene were at 84°C, 86°C, 79°C, 85°C, and 89°C, respectively.