Table 3.
Primer sequences of candidate SNP sex markers used in for SNaPshot and HRM analysis in pistachio
| No | Marker name | Primer | Position | Sequence (5′-3′) | Product size (bp) |
|---|---|---|---|---|---|
| 1 | SNP-PIS-112277 | Forward | TTACAGACACAAAACCATGACAA | 50 | |
| Reverse | GCATGCTGAATTTTCTTCCT | ||||
| Single base extension | 61-R | GCATGCTGAATTTTCTTCCTT | |||
| 2 | SNP-PIS-127343 | Forward | TCACCAATATTTTACTGCAA | 56 | |
| Reverse | CCATTTTCACTCACCCTGTC | ||||
| Single base extension | 27-S | CACCCTGTCATTACATTCTTA | |||
| 3 | SNP-PIS-133396 | Forward | GCAAACCGCAAAGAAGATTA | 52 | |
| Reverse | ACTGAAACTTGAAGATCATGGA | ||||
| Single base extension | 46-S | CAAACCGCAAAGAAGATTAAAGTA | |||
| 47-Y | GTACTGAAACTTGAAGATCATGGATCA | ||||
| 4 | SNP-PIS-135862 | Forward | GGTTTTGTGTCTGAATGTGGA | 64 | |
| Reverse | CCATGCACATTTCCCATAAT | ||||
| Single base extension | 40-Y | GTCTGAATGTGGATAATATATGG | |||
| 41-Y | TTCCCATAATCAACATGAGGC | ||||
| 5 | SNP-PIS-136404 | Forward | GAATTCTTTTAGGGGTTGTCA | 67 | |
| Reverse | CCAGCTTTAGAGTTGGCAAT | ||||
| Single base extension | 23-K | GAATTCTTTTAGGGGTTGTCAAA | |||
| 6 | SNP-PIS-167992 | Forward | CGAAAAATAACTTCATAGCGTGA | 69 | |
| Reverse | TGATCAATTGCAGCTTAGGG | ||||
| Single base extension | 36-W | AGCTTAGGGTTGCGGTTA | |||
| 7 | SNP-PIS-174431 | Forward | AGTCTCTAGCCGCGTACATA | 64 | |
| Reverse | AACTTAACAATTTYTCCACTTTCAC | ||||
| Single base extension | 56-M | CCGCGTACATATAAAAATTAACTACTCA |