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. 2015 Feb 14;15:31. doi: 10.1186/s12866-015-0343-6

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© LeGrand et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Complementation of GY448 with csrA restores the Yex phenotype and motility. GY123 (WT), GY448 (csrA), GY6536 (VC) and GY6535 (csrA/csrA+) strains were examined. A) The Yex phenotype was examined by determining phospholipase activity using a modified radial-diffusion assay. Individual colonies were streaked for isolation onto PLA indicator medium. Subsequently, phospholipase activity was detected as a bright white zone of precipitation emanating from isolated colonies. Representative images from three independent experiments. B) Phenotypic assays for motility were initiated by spotting a small portion of a colony at the center of plates containing TYE medium with 0.3% agar. Subsequently, motility was scored positive if the strains exhibited growth and migration emanating from the point of inoculation. Representative images from three independent experiments.