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. 2015 Feb 14;15:31. doi: 10.1186/s12866-015-0343-6

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© LeGrand et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of CsrA on expression of genes within the regulatory hierarchy controlling YplA export and motility. LacZ production from the vector pFUSE was driven by the upstream region, including the Shine-Dalgarno sequence, of flhDC, fliA, fleB or yplA in strains GY123 (WT), GY448 (csrA), GY6536 (VC) and GY6535 (csrA/csrA+). Bacterial cells were harvested and assayed for β-galactosidase activity. Results are averages ± standard deviation of at least three independent experiments performed in triplicate.