Table 2.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| Y. enterocolitica strains | ||
| JB580v | Serogroup O:8, Nalr ΔyenR (R−, M+) | [42] |
| GY448 | csrA::TnMod-RKm’ | [3] |
| GY6536 | csrA::TnMod-RKm’ pTM100 | This study |
| GY6535 | csrA::TnMod-RKm’ csrA::pTM100 | This study |
| GY6575 | csrA::TnMod-RKm’ flhDC::pFUSE | This study |
| GY6576 | csrA::TnMod-RKm’ fleB::pFUSE | This study |
| GY6577 | csrA::TnMod-RKm’ fliA::pFUSE | This study |
| GY6578 | csrA::TnMod-RKm’ yplA::pFUSE | This study |
| GY6579 | csrA::TnMod-RKm’ csrA::pTM100 flhDC::pFUSE | This study |
| GY6581 | csrA::TnMod-RKm’ csrA::pTM100 fleB::pFUSE | This study |
| GY6582 | csrA::TnMod-RKm’ csrA::pTM100 fliA::pFUSE | This study |
| GY6583 | csrA::TnMod-RKm’ csrA::pTM100 yplA::pFUSE | This study |
| GY6584 | csrA::TnMod-RKm’ pTM100 flhDC::pFUSE | This study |
| GY6585 | csrA::TnMod-RKm’ pTM100 fleB::pFUSE | This study |
| GY6586 | csrA::TnMod-RKm’ pTM100 fliA::pFUSE | This study |
| GY6587 | csrA::TnMod-RKm’ pTM100 yplA::pFUSE | This study |
| E. coli strains | ||
| DH5α | F− φ80lacZ∆M15 ∆(lacZYA-argF)U169 recA1 endA1 hsdR17(rk −, mk +) phoA supE44 thi-1 gyrA96 relA1 λ− | [43] |
| S17-1 λpir | recA thi pro hsdR − hsdM + RP4::2-Tc::Mu::Km Tn7 λpir (Tpr Strr) | [44] |
| Plasmids | ||
| pCR-Blunt II-TOPO | Kmr | Invitrogen |
| pFUSE | Cmr, mob +, oriR6K, suicide vector for transcriptional fusions to lacZYA | [45] |
| pTM100 | mob+, derivative of pACYC184, Cmr Tetr | [46] |
| pGY1298 | pTM100 with a 0.5-kb fragment containing csrA not directional to cat promoter | This study |
| pGY714 | pFUSE with flhDC promoter fragment | [47] |
| pGY716 | pFUSE with fleB promoter fragment | [47] |
| pGY715 | pFUSE with fliA promoter fragment | [47] |
| pGY713 | pFUSE with yplA promoter fragment | [2] |