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. 2015 Feb 15;13:11. doi: 10.1186/s12964-015-0090-1

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© Dwyer et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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A functional siRNA library screen identifies 6 putative GEF downstream of vGPCR. A. Schematic of GEF siRNA permeability screen. B. The fluorescence intensity was determined for each targeting sequence (2 sequences/target) and expressed as means of the ratio to control siRNA-transfected cells (4 non-silencing control sequences). Shared hits between vGPCR and IL-8 screens are highlighted in red. C. A two-step screen was performed; the first examined vGPCR-driven permeability. Hits from that screen were then tested for involvement in IL-8-driven permeability. The six GEFs that significantly decreased permeability when silenced are indicated.