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. 2015 Feb 18;15:34. doi: 10.1186/s12866-015-0357-0

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© Dai et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DegQ is required for optimal growth of strain MR-1 under high temperature. Genetic complementation by plasmid-borne degQ rescued bacterial growth of the degQ mutant. The MR-1ΔdegQ strains carrying empty vector (labeled as MR-1ΔdegQ) and pHERD30T-degQ plasmid (MR-1ΔdegQ+degQ) and the pHERD30T-carrying wild type MR-1 (MR-1) strains were grown in LB broth supplemented with 15 μg/ml of gentamycin. Bacterial strains were grown at 35°C.