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. 2015 Feb 12;15:51. doi: 10.1186/s12879-015-0768-4

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© Monteleone et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of miRNA-29a/b/c levels in HIV-1-infected patients (n = 58) and gender- and age-matched healthy donors (HD) (n = 21). MiRNA-29a/b/c levels were analysed in PBMC collected from HIV-1-infected patients and from healthy individuals using real time RT-PCR assays. MiRNA-29a/b/c levels are expressed as relative expression [ΔCt method] normalized to the levels of the constitutively expressed RNU6B. The relative quantity of miRNA-29a/b/c levels was calculated by the equation 2 − (ΔCt). Differences between HIV-1-infected patients and healthy donors in terms of miRNA-29 levels were analysed using the Mann–Whitney test. (°HIV-1-infected patients vs healthy donors: miRNA-29a, p = 0.251; miRNA-29b, p < 0.0001. miRNA-29c, p = 0.164). Kruskal-Wallis test was used to evaluate differences in expression among miRNA-29a/b/c in HIV-1-infected individuals and healthy subjects (*HIV-1-infected patients: p < 0.001; **healthy donors: p < 0.001).