Table 3.
Comparison of putative cathepsin L orthologs in T. molitor and T. castaneum , and comparison of key residues to those in human cathepsin L1 (NP_001903) and L2 (AAI10513)
| Substrate binding site | Substrate binding site | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Key residues 1 | Key residues | ||||||||||
| S1 | S2 | S1 | S2 | ||||||||
| 23|65 | 67|68 | 133 | 157 | 205 | 23|65 | 67|68 | 133 | 157 | 205 | ||
| Cathepsin L1 human | GG | LM | A | M | A | GG | LM | A | M | A | |
| Cathepsin L2 human | GG | FM | A | L | A | GG | FM | A | L | A | |
| Tenebrio molitor | Tribolium castaneum | ||||||||||
| TmL13 | GG | WM | A | L | A | NP_001164001 | GG | WM | A | L | A |
| TmL1 | GG | YM | A | L | Q | NP_001164314 | GG | WM | A | L | A |
| TmL2 | GG | LM | A | L | E | XP_970644 | GG | LM | A | L | Q |
| TmL3 | GG | LM | A | L | E | XP_970951 | AG | LM | A | V | Q |
| TmL29* | GG | LT | A | L | S | XP_970773* | GG | HA | T | L | S |
| TmL30* | GG | SI | A | L | D | ||||||
| TmL4 | GG | WM | A | F | K | ||||||
| TmL5 | GG | WM | A | F | V | XP_971698 | GG | WM | A | F | K |
| TmL6 | GG | WM | A | F | K | XP_971867 | GG | WM | A | F | K |
| TmL15 | GG | WM | A | F | Q | XP_971752 | GG | YL | S | K | R |
| XP_971975 | GG | WM | A | L | H | ||||||
| XP_969833 | GG | WI | A | L | H | ||||||
| TmL11 | GG | ED | G | L | T | NP_001164088 | GG | ED | A | L | T |
| TmL7 | MQ | LD | T | F | I | ||||||
| TmL8 | MQ | LD | T | F | R | ||||||
| TmL9 | LE | ME | I | Y | Y | ||||||
| TmF10 | GG | LM | A | L | P | XP_008195656 | GG | LM | A | L | P |
| TmO12 | GG | DV | A | L | E | XP_970512 | GG | DI | A | L | E |
| XP_001814509 | GG | SL | S | V | Y | ||||||
1Papain numbering.
*Predicted homolog.