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. 2015 Feb 18;15:66. doi: 10.1186/s12885-015-1073-8

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© Jagarlamudi et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Molecular forms of serum TK1 analyzed using size-exclusion chromatography. (A) Thymidine kinase 1 activity in serum fractions from an MDS patient. (B) Western blotting analysis of MDS serum fractions using the immunoaffinity method. (C) Thymidine kinase 1 activity in serum fractions from a patient with breast cancer. (D) Western blotting analysis of serum fractions from a patient with breast cancer using the immunoaffinity method. (E) Thymidine kinase 1 activity in serum fractions from a patient with prostate cancer. (F) Western blotting analysis of serum fractions from a prostate cancer patient using the immunoaffinity method. (G) Thymidine kinase 1 activity in fractions from the serum of a blood donor. The numbers denote FPLC fractions. Arrows denote the elution position with respect to molecular weight markers.