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. Author manuscript; available in PMC: 2015 Feb 22.
Published in final edited form as: Dev Biol. 2013 May 9;380(1):49–57. doi: 10.1016/j.ydbio.2013.05.003

Figure 3.

Figure 3

Tgfβ fails to activate Arf, Ink4b and Cdkn1a (Cip1). Quantitative analysis of Arf (A), Ink4b (B), Cip1 (C) and Pai-1 (D) by RT-PCR using total RNA isolated from chr4Δ70kb/Δ70kb and wild type MEFs exposed to vehicle (V) or Tgfβ (Τ) for 48 hours. Differences in transcript level between Tgfβ- and vehicle-treated wild type MEFs are significant [p <0.05 (*)]. (E, F) Quantitative analysis of representative chromatin immunoprecipitation (ChIP) assays of using chr4Δ70kb/Δ70kb and wild type MEFs exposed to vehicle (V) or Tgfβ (T) for 24 hours. ChIP assay was carried out using antibodies specific to (E) Smad2/3, (F) RPol II; expressed relative to IgG and normalized against vehicle control. Immunoprecipitated and input DNA were amplified with primers specific for the proximal Arf promoter. *, p <0.05 for Tgfβ versus corresponding vehicle.