Skip to main content
. Author manuscript; available in PMC: 2015 Feb 22.
Published in final edited form as: Dev Biol. 2013 May 9;380(1):49–57. doi: 10.1016/j.ydbio.2013.05.003

Figure 4.

Figure 4

Cis elements in the 70kb CAD interval are required for Arf induction by Tgfβ but not other stimuli. (A) Schematic diagram showing potential cis and trans activation of β-galactosidase and Arf mRNA, respectively, in ArflacZ/+, Chr4+/Δ70kb MEFs treated with Tgfβ. (B and C). β-galactosidase activity (B) and qRT-PCR analysis of Arf mRNA (C) in ArflacZ/+, chr4+/Δ70kb and Arf+/+, chr4+/Δ70kb MEFs. mRNA expression, normalized to Gapdh, expressed relative to that in vehicle treated ArflacZ/+, Chr4+/Δ70kb MEFs. (D) Oncogenic signal from human H-RASV12 induce p19Arf in chr4Δ70kb/Δ70kb MEFs. Representative western blot for the indicated proteins using lysates from chr4Δ70kb/Δ70kb and wild type MEFs after transduction with Gfp- or H-RASV12-expressing retrovirus. (E) qRT-PCR analysis of Arf mRNA using chr4Δ70kb/Δ70kb and wild type MEFs from different passage. * and **, p <0.05 for P7 versus P2 in chr4Δ70kb/Δ70kb and wild type, respectively. (F) Cell counting assay of chr4Δ70kb/Δ70kb and wild type MEFs from different passage after cultured for 6 days. *, and ** p <0.05 for chr4Δ70kb/Δ70kb versus wild type for P2 and P9, respectively.