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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Arch Biochem Biophys. 2014 Mar 13;548:74–85. doi: 10.1016/j.abb.2014.02.014

Fig. 2. NaN3 inhibition of Compound I.

Fig. 2

A, Stopped flow analysis of MPO (23 nM) without inhibitor (a), in the presence of NaN3 (30 μM) after pre-incubation for 1 hr prior to triggering H2O2 addition (b) and after equilibrium dialysis (c). B, Lineweaver-Burk plots of for NaN3 inhibition on MPO calculated from resorufin production data. Titrations of 0, 7.5, 30 μM NaN3 are shown for the different concentration of ADHP from 25, 50, 100, 200 and 400 μM. Titrations were performed and analyzed as described under “Experimental Procedures.”