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. Author manuscript; available in PMC: 2015 Apr 15.
Published in final edited form as: Arch Biochem Biophys. 2014 Mar 13;548:74–85. doi: 10.1016/j.abb.2014.02.014

Fig. 3. Comparison of Compound I inhibition by NaN3 and 4-ABAH using simultaneous reaction modeling.

Fig. 3

A, Fluorescence progress curves presented in arbitrary units (au) for various indicated concentration of NaN3 were globally fit to a one-step slow-tight binding model detailed in Scheme I. Representative goodness-of-fit for the 30 μM ABAH with 15 μM ADHP reaction is shown as a residual plot. B, Similar plots for 4-ABAH analyzed by a two-step slow-tight binding model (Scheme II) for indicated concentrations of 4-ABAH to accommodate the biphasic phenotypic of the reaction. Representative goodness-of-fit for 0.64 μM ABAH with 16 μM ADHP are also shown. Titrations were performed and analyzed as described under “Experimental Procedures.”