Fig. 4. Reversibility of 4-ABAH-mediated MPO inhibition.
A, Stopped flow analysis of MPO (23 nM) and ABAH were mixed and incubated in assay buffer for 1 hr prior to triggering H2O2 addition. B, Exhaustive dialysis of identical mixtures into assay buffer followed addition with H2O2. Titrations were performed and analyzed as described under “Experimental Procedures.”