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. Author manuscript; available in PMC: 2015 Feb 22.
Published in final edited form as: Methods Enzymol. 2014;538:49–65. doi: 10.1016/B978-0-12-800280-3.00004-9

Figure 4.2.

Figure 4.2

Metabolic phenotypes of newly differentiated human adipocytes. Lipolytic rates, basal and in response to isoproterenol (Iso), norepinephrine (NE), or 8-bromo-cAMP (A) and in response to insulin (B), are measured on d14 of differentiation. Basal lipolysis was measured in the conditions of 1 units/ml ADA +20 nM PIA. Inhibition of lipolysis by insulin was measured against 8-bromo-cAMP (1 mM)-stimulated conditions. (C) The rates of 14C-2-deoxy-glucose uptake were measured after 3–4 h of starvation at 0, 60, 120, 600, and 3000 pM insulin concentrations. Data are obtained from sc ASCs derived from five independent subjects. Some of these data have been published (Lee & Fried, 2012; Lee et al., 2012).