Final concentrations	Stock concentrations	Amount/l
DMEM/F12, 18.5 mM glucose			1 pack
HEPES	15 mM		3.57 g
NaHCO3	25 mM		2.22 g
Penicillin/streptomycin	100 units/ml (pen) and 100 μg/ml (strep)	10,000 units/ml (pen) and 10,000 μg/ml (strep)	10 ml
d-Biotin	33 μM	3.3 mM	10 ml
Pantothenate	17 μM	1.7 mM	10 ml
Dexamethasone	100 nM	10 mM	10 μl
Insulin	100 nM	600 μM	167 μl
Rosiglitazone or other TZDs	1 μM	10 mM	100 μl
IBMX	0.5 mM		110 mg
T3	2 nM	20 μM	100 μl
Transferrin	10 μg/ml	10 mg/ml	1 ml