Final concentrations | Stock concentrations | Amount/l | |
---|---|---|---|
DMEM/F12, 18.5 mM glucose | 1 pack | ||
HEPES | 15 mM | 3.57 g | |
NaHCO3 | 25 mM | 2.22 g | |
Penicillin/streptomycin | 100 units/ml (pen) and 100 μg/ml (strep) | 10,000 units/ml (pen) and 10,000 μg/ml (strep) | 10 ml |
d-Biotin | 33 μM | 3.3 mM | 10 ml |
Pantothenate | 17 μM | 1.7 mM | 10 ml |
Dexamethasone | 100 nM | 10 mM | 10 μl |
Insulin | 100 nM | 600 μM | 167 μl |
Rosiglitazone or other TZDs | 1 μM | 10 mM | 100 μl |
IBMX | 0.5 mM | 110 mg | |
T3 | 2 nM | 20 μM | 100 μl |
Transferrin | 10 μg/ml | 10 mg/ml | 1 ml |