A, Table of the predicted miR-145 binding sites in the ARF6 mRNA 3’UTR as predicted by TargetScan algorithm. B, ARF6 mRNA expression in breast cancer cell lines via qRT-PCR. C, ARF6 protein expression in MDA-MB-231 cells. D, Luciferase reporter for ARF6 mRNA 3’UTR following miR-145 overexpression. E, ARF6 mRNA levels in MDA-MB-231 cells following miR-145 overexpression. F, ARF6 protein levels in MDA-MB-231 cells following miR-145 overexpression. G&H, MCF-7 cells overexpressing constitutively active ARF6. A loss of E-cadherin demonstrated via western blotting and a more invasive phenotype as evidenced by matrigel coated invasion assays. * p< 0.05