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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Mol Cancer Res. 2014 Sep 17;13(2):281–292. doi: 10.1158/1541-7786.MCR-14-0230

Fig. 2. BAs modulate cell proliferation and apoptosis through Nur77 nuclear export in a time-dependent manner.

Fig. 2

Nur77 expression and location as visualized by immunofluorescence microscopy in DMSO, DCA (150 µM) or LCA (20 µM) treated HCT116 (A) and Huh7 cells (B) at indicated times. Cells were immunostained with anti-Nur77 and anti-cleaved caspase 3 antibodies followed by species appropriate Alexa Fluor secondary antibodies. Arrows showed Huh7 cells that lacked cleaved Caspase 3 and had increased nuclear Nur77. (C) TUNEL staining was performed in LCA-treated HCT116 and Huh7 cells. The percentages of apoptotic cells were counted with apoptotic cell staining in red while DAPI as nuclear staining in blue. Bar graph shows the number of apoptotic cells in 5 random fields (40×).