Figure 4. Chemical compounds NSC-156306 and NSC-606170 affected endogenous CYP2C9 expression by altering the expression of hsa-miR-128-3p in HepaRG cells.

Differentiated HepaRG cells were treated with 0, 10 nmol/L, or 100 nmol/L NSC-156306 or NSC-606170, and harvested 48 hours after treatments. Each assay was performed from at least 3 independent experiments. * P < 0.05; ** P < 0.001. (a) Dysregulated expression of hsa-miR-128-3p. Data are shown as relative hsa-miR-128-3p levels versus U6. (b) Dysregulated expression of CYP2C9 mRNA by NSC-156306 or NSC-606170 treatment. Data are shown as relative CYP2C9 mRNA levels versus GAPDH reference. (c) and (d) Dysregulated CYP2C9 protein levels by NSC-156306 or NSC-606170 treatment.