Table 1.
Gene | Primers (forward, reverse) | Annealing temperature (°C) | Amplified product length (bp) |
---|---|---|---|
cagA | 5′-GATAACAGGCAAGCTTTTGAGG-3′ | 57 | 349 |
5′-CTGCAAAAGATTGTTTGGCAGA-3′ | |||
| |||
vacAs1 | 5′-ATGGAAATACAACAAACACAC-3′ | 52 | 259 |
5′-CTGCTTGAATGCGCCAAAC-3′ | |||
| |||
vacAm1 | 5′-CAATCTGTCCAATCAAGCGAG-3′ | 56 | 570 |
5′-GCGTCTAAATAATTCCAAGG-3′ |