Table 1.
Summary of bagasse fosmid pyrosequencing data
| Raw reads | ||||||
|---|---|---|---|---|---|---|
| Dataset | Number of sequences | Number of nucleotides | Sequence length | |||
| Average | SD | Minimum | Maximum | |||
| 1. Raw reads | 1,038,205 | 591,656,071 | 569.9 | 173.3 | 40 | 1,595 |
| 2. Read screen repeats | 982,383 | 569,556,388 | 579.8 | 164.7 | 40 | 1,595 |
| 3. Read screen repeats and trim vector | 726,980 | 421,491,438 | 579.8 | 166.0 | 40 | 1,595 |
| Assembled sequences | ||||||
| Dataset | Number of sequences | Number of nucleotides | Sequence length | |||
| Average | SD | Minimum | Maximum | |||
| 1. Contigs | 17,829 | 32,867,905 | 1,843.5 | 2,394.6 | 100 | 46,577 |
| 2. Singletons (non-redundant) | 185,543 | 109,290,202 | 589.0 | 163.5 | 40 | 1,595 |
The bagasse fosmid library was sequenced on one full lane of the 454 GS-FLX Titanium, resulting in approximately one million raw reads. The reads with contaminating sequences of vector or host genome were removed before contig assembling and redundant sequence cleaning.