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Size selection with the AMPure XP beads are calibrated for each new lot by mixing different ratios of beads with the 20 bp ladder. The products are then run the Bioanalyzer HS DNA chip to determine the cut-off sizes for different ratios.
| XP:sample vol/vol ratio |
XP bead volume |
Approx. size cut-off - bp |
|---|---|---|
| none | none | None |
| 0.6:1 | 48µL | 400 |
| 0.7:1 | 56µL | 300 |
| 0.8:1 | 64µL | 200 |
| 1.0:1 | 80µL | 175 |
| 1.2:1 | 96µL | 150 |
| 1.4:1 | 112µL | 125 |
| 1.6:1 | 128µL | 100 |
| 1.8:1 | 144µL | 100 |
| 2.0:1 | 160µL | 100 |
