Table 3.
Custom Small RNA-Seq v1.5 Primers, Adapters and qRT-PCR Primer Sequences.
| Small RNA-seq Version |
Primer | Sequence |
|---|---|---|
| Custom Illumina v1.5 | RS-TS-5’ adapter | G*TTCAGAGTTCTACAGTCCGACGATC |
| RS-TS-3’ adaptera | /5rApp/GATCGGAAGAGCACACGTCTGAACTCCAGTCAC*/3ddC/ | |
| RS-PCR2Da | A*ATGATACGGCGACCACCGACAGGTTCAGAGTTCTACAGTCCGAC | |
| RS-TS-PCR1Aa | G*TGACTGGAGTTCAGACGTGTGCTCTTCCGATC | |
| RS-TS-PCR-I-1b | G*CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC | |
| qRT-PCR Primers | 5S rRNA Forward | CGATCTCGTCTGATCTCGGAAG |
| 5S rRNA Reverse | AGGCGGTCTCCCATCCAAG | |
| 5.8S rRNA Forward | GGCTCGTGCGTCGATGAAG | |
| 5.8S rRNA Reverse | CGCTCAGACAGGCGTAG | |
| 18S rRNA Forward | CCAGTAAGTGCGGGTCATAAGC | |
| 18S rRNA Reverse | CCATCCAATCGGTAGTAGCGAC | |
| 28S rRNA Forward | TCAGACCCCAGAAAAGGTGTTG | |
| 28S rRNA Reverse | TGATTCGGCAGGTGAGTTGTTAC | |
| EEF1A1 Forward | GCCCCAGGACACAGAGACTTTATC | |
| EEF1A1 Reverse | CAACACCAGCAGCAACAATCAG | |
| U6 Forward | CGCTTCGGCAGCACATATAC | |
| U6 Reverse | TTCACGAATTTGCGTGTCAT | |
| LRP6 Forward | CGGTGAGAGAAGAGAACGCG | |
| LRP6 Reverse | GCCTCCAACTACAATCGTAGC |
a
* indicates phosphorothioate bond; /5rApp/ indicates 5’ adenylation; /3ddC/ indicates 3’ dideoxy-C
b
bold indicates the index sequence