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. Author manuscript; available in PMC: 2015 Feb 23.
Published in final edited form as: Cancer Res. 2008 Aug 1;68(15):6232–6240. doi: 10.1158/0008-5472.CAN-07-5654

Figure 1.

Figure 1

Doxycycline-inducible VEGF expression. Conditioned media were collected from cell cultures of parental MCF-7, pBTE-transfected C9, C9V4, and C9V18, treated with or without 0.2 µg/mL doxycycline (Dox) for 48 h and Western blotted with a specific antibody against human VEGF (R&D Systems). The upper band of VEGF with higher molecular weight is most likely due to the posttranslational modifications. E. coli–expressed human recombinant VEGF lacking posttranslational modifications was used as standard in the first two lanes. Each lane contains the equivalent VEGF secreted by ~500 cells over a 48-h period.