Table 1.
Continued VEGF overexpression in primary tumors results in frequent spontaneous metastases to the lung in either estrogen- or tamoxifen-treated mice
| Treatment | Cell line |
No. of mice |
No. of mice with lung metastasis |
|---|---|---|---|
| E2 | |||
| −Dox | C9V4 | 5 | 0 |
| C9V18 | 5 | 0 | |
| +Dox (0.2 mg/mL) | C9V4 | 5 | 3 |
| C9V18 | 4 | 3 | |
| +Dox to day 11 (0.2 mg/mL) | C9V4 | 6 | 0 |
| Tamoxifen | |||
| −Dox | C9V4 | 3 | 0 |
| C9V18 | 5 | 0 | |
| +Dox (0.2 mg/mL) | C9V4 | 4 | 1 |
| C9V18 | 5 | 3 |
NOTE: Incidence of mouse lung metastases was summarized for several animal studies, including the studies of Figs. 2A and 3A. Mouse lung tissues were collected from C9V4 and C9V18 xenograft–bearing mice at the end of each animal study, which typically occurred ~50 d after cell inoculation. Lung metastases were examined by X-gal staining as described in ref. 33.
Abbreviation: Dox, doxycycline.