Table 2.
Viruses made using the p2KO ablation/insertion vector
| TPV recombinant | Gene ablated | Gene added | Reporter (s) |
|---|---|---|---|
| TPV/egfp | --- | EGFP | |
| TPV/Δ66R | 66R | --- | mCherry |
| TPV/Δ66R/mGM-CSF | 66R | mGM-CSF | mCherry |
| TPV/Δ66R/mMCP-1 | 66R | mMCP-1 | mCherry |
| TPV/Δ66R/fliC | 66R | fliC | mCherry |
| TPV/Δ2L | 2L | --- | mCherry |
| TPV/2L/Δ66R/fliC | 66R, 2L | fliC | EGFP, mCherry |
All viruses expressed the fluorescent reporter mCherry with the exception of TPV/egfp, which was modified to express fluorescent reporter EGFP but had no other genetic modifications. “Gene added” refers to an ORF added and expressed other than the fluorescent reporter. The TPV/Δ2L/Δ66R/fliC double-ablated recombinant TPV expressed both EGFP and mCherry.