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. 2015 Feb 23;6:52. doi: 10.3389/fpls.2015.00052

Figure 12.

Figure 12

Experimental assessment of the 2D Stress Feedback model (SFm) of the entire morphogenetic dynamics of the SAM from Hamant et al. (2008), reprinted with permission from AAAS. (A) marking cortical microtubules (green) and cell shape (red) at the surface of a meristem generating a young primordium (P). Cortical microtubule marking is obtained using the expression of a fusion protein involving the Green Fluorescent Protein and the Microtubule Binding Domain (GFP-MBD) under the control of the constitutive promoter 35S (p35S::GFP-MBD) Scale bar, 20 mm. (B) Microtubule orientation (red bars) in cells in the 2D SFm (extracted from confocal data). Note the alignment of the virtual microtubule orientations in the boundary zone and compare to (A). (C) Simulation of an auxin-induced primordium. The 2D SFm results in orthoradial alignment of microtubules around the growing primordium. (D) Tip-growing simulation with the stress-feedback model generating a growing stem. Microtubules align mainly orthoradially in the stem, which has a regular shape.