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. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052

FIGURE 1.

FIGURE 1

Generation of the CB-Myc6-Rb1 construct. (A) cloning of the Rb1 fragment into the pCS2-CB-Myc6 vector. A 1583bp Rb1 gene product was PCR amplified using Forward and Reverse primers. The resulting Rb1 fragment was cloned between EcoRI and Xbal restriction sites of pCS2 vector containing CB-Myc6 construct (a 1012bp CB gene fragment and a hexameric Myc6 sequence tag), as described previously (Li et al., 1996, 2000). (B) The CB-Myc6-Rb1 fragment was cloned into the pTetSplice vector between the SalI and EcoRV restriction sites.