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. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052

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Copyright © 2015 Tarang, Doi, Gurumurthy, Harms, Quadros and Rocha-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DN-CBRb transgene expression in a P21 DN-CBRb⁺/ROSA-CAG-rtTA⁺ mouse cochlea. (A–F) Strong cytoplasmic and nuclear ISH signal was observed at the inner hair cells (IHCs), region of Dox-treated DN-CBRb⁺/ROSA-CAG-rtTA⁺ mouse cochlea. (A–D) Transgene expression was detected throughout the length of the cochlea, but visibly stronger at the middle and basal regions compared to the apex of the cochlea. Mosaic DN-CBRb expression was observed (arrows) and may indicate incomplete recombination in those cells. Likewise, supernumerary cells (asterisk) showed strong hybridization signal, suggesting efficient transgene transmission to the newly formed cells. (E) Sections of the cochlea counterstained with Nuclear Fast Red, confirmed our observations on whole mount preparations and allowed for a better appreciation of a supernumerary IHC (asterisk), as well as some light transgene expression in the spiral ganglion neurons (F). SGN , spiral ganglion neurons; Bar = 10 μm.