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. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052

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Copyright © 2015 Tarang, Doi, Gurumurthy, Harms, Quadros and Rocha-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunohistochemical localization of stereocilia and proliferating cells on DN-CBRb⁺/ROSA-CAG-rtTA⁺ mouse cochleae. (A–C) Phalloidin (green) staining of P10 CBRb⁺/ROSA-CAG-rtTA⁺ mouse cochlea revealed the presence of F-actin-positive stereocilia-like structures (arrows). Due to the peculiar cochleae anatomy, (B,C) were rotated to facilitate visualization of the stereocilia. (D) EdU (green) labeling in DN-CBRb⁺/ROSA-CAG-rtTA⁺ (CBRb) at P10 showed no proliferation within the OC; however, a few EdU-positive cells were observed at the greater epithelial ridge. Asterisks indicate supernumerary cells. DAPI = Blue; Myosin VIIa = Red. Bar = 10 μm.