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Genotyping of the DN-CBRb mouse model. PCR with primers specific for the CB-Rb1 fusion transgene resulted in a 401 bp band in the DN-CBRb-positive animals (lanes 2, 4, and 5), but not in those lacking the transgene (lanes 1 and 3). rtTA genotype was carried following the protocol available at the Jackson Laboratories website (http://jaxmice.jax.org/strain/006965.html). (+) = Control DNA showing the transgene. (-) = Control PCR not containing DNA. M = 100 bp DNA ladder.
