FIGURE 4.

Dox-mediated DN-CBRb transgene activation increases cell proliferation. HEI-OC1 cells co-transfected with purified pTet-Splice-DN-CBRB and the pCMV-Tet3G vectors were cultured in the absence (-) or presence (+) of Dox. Cell proliferation was determined 48 h after transfection using CYQUANT NF Proliferation Assay. Percentage change in proliferation was calculated using change in fluorescence values with untransfected cells as control. A modest, but significant increase in cell number was observed on transfected cells following Dox treatment. In contrast, no significant changes were observed between transfected HEI-OC1 cells not treated with (-Dox) and the untransfected control. *P < 0.05.