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. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052

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Copyright © 2015 Tarang, Doi, Gurumurthy, Harms, Quadros and Rocha-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dox-mediated DN-CBRb transgene activation has no effect on active caspase 3 activation. HEI-OCI cells co-transfected with TetO-DN-CB-myc6-Rb1 and the pCMV-Tet3G transactivator vector were cultured in the absence (-) or presence (+) of Dox. (A) Protein expression of active caspase 3 in the absence or presence of Dox for 24 and 48 h. (B–D) Immunohistochemical analysis of apoptotic cell death by CaspaTag assay. (B) Untransfected cells. (C) Transfected cells treated with Dox. (D) Untransfected cells treated with Staurosporine (positive control). Arrows point to apoptotic cells. S = Untransfected cells treated with Staurosporine for 4 h ; U = Untransfected cells; 1 = Transfected cells treated with Dox only for 48 h; 2 = Transfected cells not treated with Dox for 24 h; 3 = Transfected cells treated with Dox for 24 h; 4 = Transfected cells not treated with Dox for 48 h; 5 = Transfected cells treated with Dox for 48 h. Bar = 10 μm.