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. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052

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Copyright © 2015 Tarang, Doi, Gurumurthy, Harms, Quadros and Rocha-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dox-mediated DN-CBRb activation is tightly regulated by Dox administration. Cochleae of Dox treated (lanes 1–3) and untreated (lanes 4–6) P10 DN-CBRb⁺/ROSA-CAG-rtTA⁺ mice were submitted to Western blot analysis with anti-RB1 antibody. Consistent with a tight regulation of the DN-CBRb expression by Dox administration, no endogenous RB1 expression was observed on DN-CBRb⁺/ROSA-CAG-rtTA⁺ mice cochleae in the presence of Dox.