Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Tarang, Doi, Gurumurthy, Harms, Quadros and Rocha-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

DN-CBRb transgene activation in the inner ear of postnatal (P) 36 DN-CBRb⁺/ROSA-CAG-rtTA⁺ (CBRb⁺) and DN-CBRb^-/ROSA-CAG-rtTA⁺ (CBRb^-) mice after 3 (group 1), 7 (group 2), and 10 (group 3) days of Dox treatment. (A–C) Anti-RB1, which reacts with both hyperphosphorylated and hypophosphorylated forms of RB1, as well as anti-c-myc antibodies were used for detection. Densitometric analysis was done using ImageJ software (http://imagej.nih.gov/ij/). The corresponding values obtained were normalized to the negative control CBRb (-) and then to β-actin. The relative RB1 expression levels are shown underneath each blot. (D) Graphic representation of normalized RB1 expression levels plotted against the different treatments highlights a consistently lower RB1 detection in CBRb⁺ samples. Each lane on the Western blot gel (A–C) and each column on the graphic (D) correspond to a different individual and sample. (-) = CBRb^-; *P < 0.05.