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. 2015 Feb 23;9:52. doi: 10.3389/fncel.2015.00052

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Copyright © 2015 Tarang, Doi, Gurumurthy, Harms, Quadros and Rocha-Sanchez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Spatial analyses of the DN-CBRb transgene activation in P18 DN-CBRb⁺/ROSA-CAG-rtTA⁺ and DN-CBRb⁺/ROSA-CAG-rtTA^- mice heart, eye, lungs, and cochlea. (A) Confirming efficiency of Dox-mediated transgene activation, expression of the endogenous RB1 was downregulated in the CBRb⁺/ROSA-CAG-rtTA⁺ (+) tissues, but not in the CBRb^-/ROSA-CAG-rtTA⁺ (-) control mice tissues. Densitometric analysis was performed as described in Figure 4. The relative RB1 expression levels are shown underneath each blot. (B) Graphic representation of normalized RB1 expression levels plotted against the different tissues. Inter-individual variation on endogenous RB1 levels may explain differences in individual responses to transgene activation and RB1 downregulation. (C) RT-PCR analyses in eye, heart and cochleae revealed significant upregulation of the DN-CBRb transcript in dox-treated DN-CBRb⁺/ROSA-CAG-rtTA⁺ tissues, but not in DN-CBRb⁺/ROSA-CAG-rtTA^-. CBRb⁺/rtTA⁺ = DN-CBRb⁺/ROSA-CAG-rtTA⁺; CBRb^-/rtTA⁺ = DN-CBRb^-/ROSA-CAG-rtTA⁺; CBRb⁺/rtTA^- = DN-CBRb⁺/ROSA-CAG-rtTA^-. *P < 0.05.