Table 1.

Oligonucleotides used in this work.

Primer name	Sequence (5′-3′)a	Gene amplified	GenBank accession number
Tasm1OE-f	GCTCTAGAATGCAACTGTCCAACATCTTCACTC	epl1	AJ901879.1
Tasm1OE-r	CCAATGCATTTAGAGACCGCAGTTCTTAACAGG	epl1	AJ901879.1
Tvsm1OE-f	GCTCTAGAATGCAGTTCTCCAGCCTCTTCAAG	sm1	DQ121133.1
Tvsm1OE-r	CCAATGCATTTAGAGGCCGCAGTTGCTCACAGC	sm1	DQ121133.1
Tasm1KO5′-f	CGGGATCCGCACTGGGTAGATGCTGGTCTG	epl1	AJ901879.1
Tasm1KO5′-r	CTCCTTCAATATCAGTTAACGTCGATCCTGAGTAGTGAAGCGAATGTGCTG	epl1	AJ901879.1
Tasm1KO3′-f	CAGCACTCGTCCGAGGGCAAAGGAATAGCGGAGCAATGTAAGCAGATCGAC	epl1	AJ901879.1
Tasm1KO3′-r	CCGCTCGAGCCTTACTGCAAAGGGTCTGGATGC	epl1	AJ901879.1
Tvsm1KO5′-f	GCTCTAGAACAATGCCGGTAGTACACCGTTCG	sm1	DQ121133.1
Tvsm1KO5′-r	CTCCTTCAATATCAGTTAACGTCGATCGGGTACAGCAAACTGACTCGTCAC	sm1	DQ121133.1
Tvsm1KO3′-f	CAGCACTCGTCCGAGGGCAAAGGAATAGCGACCAGTAAACCGCCATTCATCG	sm1	DQ121133.1
Tvsm1KO3′-r	CCGCTCGAGGGACTTGTCGAATTTCCCATCTCG	sm1	DQ121133.1
Ta-TEF-1-Fw	AGGCCGAGCGTGAGCGTGGTAT	ef-1 alpha	ID 146236
Ta-TEF-1-Rv	ATGGGGACGAAGGCAACGGTCTT	ef-1 alpha	ID 146236
hph-f	GATCGACGTTAACTGATATTGAAGGAG	hph	X03615.1
hph-r	CTATTCCTTTGCCCTCGGACGAGTGCTG	hph	X03615.1
GLUA-F	GTGAAGCTGGTTTGGGAAATG	SlGLUA	M80604.1
GLUA-R	TTGCCAATCAACGTCATGTCTAC	SlGLUA	M80604.1
PR-5-F	GGTGCCAGACTGGTGATTGTG	SlPR-5	AY093595.1
PR-5-R	TTGGTGGTTTACCCCATCCTT	SlPR-5	AY093595.1
DOX1-F	TCACACCATAGATTGGACTGTTCA	Slα-DOX1	AY344539.1
DOX1-R	GGCACGCATTCCTGCAA	Slα-DOX1	AY344539.1
CHI9-F	AACGCGGGAATTGTTCGA	SlCHI9	Z15140.1
CHI9-R	GCAGGACATGCGTCATTGTT	SlCHI9	Z15140.1
TLRP-F	TGCGGTGAAATTGGATAACG	SlTLRP	X77373.1
TLRP-R	GCCATAGCCCTTGCCATAATAA	SlTLRP	X77373.1
CEVI16-F	AACGGAGATGGCTCGAAGCGTG	SlCEVI16	X94943.1
CEVI16-R	CATCGGTCCACAATATCTGGTCTG	SlCEVI16	X94943.1
ACT-f	GCTGCAGGTATCCACGAGACTACC	SlTOM51	U60481.1
ACT-r	GAT TTC CTT GCT CAT ACG GTC AGC	SlTOM51	U60481.1

^a Restriction sites are indicated as underlined letters.