Fig. 3.

Hippocampal nitrite, S-nitrosothiol and cGMP levels. Hippocampi were rapidly excised from sham or post-arrest animals randomized to nitrite or placebo upon infusion completion. (A) A representative tracing of data output from the nitric oxide analyzer demonstrates the increased area under the curve for similar volumes of hippocampal homogenate from nitrite treated animals compared to placebo. The summary data for eight animals is shown graphically below (B). Representative curves from samples pre-treated with acidified sulfanilamide (C) demonstrate the increase in S-nitrosothiols after nitrite therapy and are likewise summarized below (D). Significant increases in nitrite (B) and S-nitrosothiol (D) content were noted in the brains of nitrite-treated compared to placebo and sham animals. (E) Similarly timed hippocampal homogenates analyzed for cGMP content did not reveal significant differences. All results normalized to protein content. * Denotes p < 0.05 and ^# denotes p < 0.01 by ANOVA.