FIG. 4.
TUNEL analyses of quadriceps of Cre-mediated recombination from Cre-ER™-VCPR155H/+ mice. TUNEL staining of quadriceps muscles from corn oil (CO)-treated (a) WT and Cre-ER™-VCPR155H/+ mice, and tamoxifen (TM)-treated WT and VCPR155H/+ mice at 18 months of age (magnification: 630×). Arrows indicate TUNEL+ cells. Scale bar represents 250 μm. (b) Quantification of TUNEL+ cells in corn oil (vehicle)- and tamoxifen-treated Cre-ER™-VCPR155H/+ mice. (c) Western blot analysis of apoptosis markers, Bcl-2, BAX, and Puma in 18-month-old WT and Cre-ER™-VCPR155H/+ tamoxifen-treated animals. β-Actin was used as a loading control for these samples. (d) Densitometry analysis confirmed levels of the apoptosis markers. Statistical significance is denoted by *p<0.05 and **p<0.005 with one-way ANOVA. The number of animals used was n=3/group. Color images available online at www.liebertpub.com/hgtb