FIG 2.

pDC stimulated with partially purified HCV strain Jc1 do not mount IFN-α responses. (A) The 50% tissue culture infective doses (TCID₅₀) of a crude Jc1 preparation, partially heparin column-purified Jc1, and the concentrated flowthrough (FT conc.). (B) pDC were stimulated with crude preparations of Jc1 (Jc1), partially heparin column-purified Jc1, or the flowthrough of the heparin column purification of Jc1 at an MOI of 1. After 18 h, IFN-α production was assessed in the supernatant by an ELISA method (pDC, n = 3). (C) TCID₅₀ of a crude Jc1 preparation, ultracentrifuged Jc1 (Jc1 UZ), and Jc1 ultracentrifuged through a sucrose cushion (Jc1 UZ+C). (D) pDC were stimulated with crude preparations of Jc1 or Jc1 that was collected after ultracentrifugation with or without a sucrose cushion (pDC, n ≥ 4). The error bars indicate SEM.