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. 2014 Dec 31;89(6):3026–3037. doi: 10.1128/JVI.03432-14

FIG 1.

FIG 1

Creation of HB29 S- and M-based minigenome constructs. (A) Schematic of the genome organization of S- and M-based minigenome constructs and the relative orientation of the open reading frames in the transcription plasmids pTVT7. (B) Effect of increasing amounts of pTM1-HB29N on M-segment minigenome assay. BSR-T7/5 cells were transfected with pTVT7-HB29M:hRen, pTM1-HB29ppL, pTM1-FF-Luc, and the indicated amount of pTM1-HB29N. (C) Effect of increasing amounts of pTM1-HB29ppL on M-segment minigenome assay. BSR-T7/5 cells were transfected with pTVT7-HB29M:hRen, pTM1-HB29N, pTM1-FF-Luc, and the indicated amount of pTM1-HB29ppL. In both cases, empty pTM1 vector was used to ensure that the total amount of DNA used in each transfection was the same (2.51 μg). Renilla luciferase activity was measured 24 h posttransfection.