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Oligonucleotides used for 3′ RACE analysis
| Primer for: | Sequence (5′–3′)a | Genome position (nt) (genomic RNA) |
|---|---|---|
| S segment | ||
| S-PCR (G) | GATCTCAGGTAACCCAAGTCTAAGCCTC | 518–545 |
| S-PCR sequence (G) | TCAACGAGGTCTCTCCTGAGAGTGGGGTAC | 116–145 |
| S-PCR (AG) | GCGCATCTTTACATTGATAGTCTTGGTGAAGGC | 1346–1373 |
| S-PCR sequence (AG) | CTGCAGCAGCACATGTCCAAGTGGGAAG | 1146–1178 |
| RACE-AP | GACCACGCGTATCGATGTCGAC | NAb |
| Oligo(dT) | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV | NA |
V is nucleotide A, C, or G.
NA, not applicable.
