FIG 2.

Mutations in the KD and 15-aa motifs of TBSV p92. (A) A schematic representation of the TBSV p92 protein is shown with the relevant motifs indicated. Residues selected for mutational analysis are indicated in boldface and are numbered according to their corresponding genomic mutant in the CG series. All mutants shown above the sequence contained alanine substitutions, whereas those below contained alternate substitutions, which are specified in parentheses. (B) Northern blot analysis of viral RNA accumulation in protoplasts inoculated with the genomic CG mutants. The identities of the mutants analyzed are provided above each lane, and the positions of subgenomic (sg) and genomic (g) viral RNAs are indicated to the left. The accumulations of plus strands (+) are shown in the upper panel, while those of minus strands (−) appear in the lower panel. (C) Quantification of accumulation levels of plus (black bars) and minus (white bars) sense viral RNAs from CG mutant infections. In the graphs, absolute accumulation values of the genomic RNAs are compared, whereas, for sg mRNAs, the ratios of sg mRNA levels to their cognate genomic RNA levels are compared. Relative accumulation levels are presented with standard errors derived from three independent experiments.