FIG 4.

Summary of the impact of TBSV CG and KJ mutants on viral RNA accumulation. (A) All substitutions in the KD, 15-aa, and 19-aa motifs tested in TBSV mutants are indicated. (B, C) Diagrammatic representations of TBSV genome replication and sg mRNA transcription showing the different steps in which mutants affected plus-strand (solid black lines) or minus-strand (dotted gray lines) production. The effects are grouped into three categories: large (red), in which relative accumulation levels were between 0 to 33% of that of wt; medium (blue), levels between 34 to 66% of wt and; small (black), levels greater than 67% of wt.