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CG and KJ mutations in nonreplicating TBSV genomes affect amplification of a DI RNA. (A, C) Northern blot analysis of DI RNA accumulation in protoplasts coinoculated with nonreplicating genomic CG or KJ mutants and a DI RNA. (B, D) Relative accumulation levels of DI RNA from panels A and C are shown by the black bars and compared with corresponding levels of genomic RNA for replication-competent mutant genomes (gray bars) derived from Fig. 2C and 3C, respectively.
