FIG 6.

Complementation of pp71 HCMV mutant ADsubUL82 by ICP0. (A) Analysis of expression of myc-tagged pp71 before and after induction of HFT-mycpp71 cells with doxycycline for 24 h compared to control HFT cells and wt HCMV TNwt-infected HFT cells, as indicated. The difference in mobility of pp71 in the induced and infected cells is due to the presence of the myc tag in the former. m, mock infected. (B) Comparison of the relative plaque-forming efficiencies of HCMV ADsubUL82 in induced HFT, HFT-mycpp71, and HFT-ICP0 cells, expressed as fold increase over that in control HFT cells. (C). Comparison of IE1, IE2, pp71, UL44, and pp28 expression during wt HCMV strain AD169 and ADsubUL82 (Δpp71) HCMV infection of induced HFT-ICP0 cells, as indicated, confirming the absence of pp71 expression by ADsubUL82 and illustrating similar levels of expression of UL44. (D) Western blot analysis of IE1, pp71, and UL44 expression after infection by HCMV ADsubUL82 in uninduced and induced HFT-ICP0 cells.