FIG 2.

Identification of cellular proteins associated with the MuV P protein by FOS-tagged purification. (A) Overview of the FOS-tagged purification of cellular proteins associated with the MuV N and P proteins. (B) Vero cells transfected with pCAG-N-FOS and/or pCAG-P-FOS were immunostained at 24 h posttransfection with mouse anti-N MAb and rabbit anti-V/P PAb, followed by AF488-conjugated anti-mouse IgG and AF594-conjugated anti-rabbit IgG. The cell nuclei were stained with DAPI (blue). (C) Purified products were subjected to SDS-PAGE, followed by CBB staining. The empty vector (EV) was used as a negative control. Lane M is a protein molecular mass marker. (D) Purification of the P-FOS protein was confirmed by immunoblotting using mouse anti-FLAG MAb (M2) and rabbit anti-V/P PAb. (E) Detection of Hsp70 family proteins in the purified P-FOS complexes was performed by immunoblotting using the appropriate antibodies.