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. 2014 Dec 31;89(6):3188–3199. doi: 10.1128/JVI.03343-14

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FIG 4 — Determination of the regions responsible for the interaction between the P protein and Hsp72. (A) FLAG-Hsp72 and its deletion mutants were coexpressed with HA-P in 293T cells, immunoprecipitated with anti-FLAG antibody, and immunoblotted with anti-FLAG and anti-HA antibodies. (B) HA-P and its deletion mutants were coexpressed with FLAG-Hsp72 in 293T cells, immunoprecipitated with anti-HA antibody, and immunoblotted with anti-HA and anti-FLAG antibodies. (C) FLAG-Hsp72 was coexpressed with HA-V in 293T cells, immunoprecipitated with anti-HA antibody, and immunoblotted with anti-HA and anti-FLAG antibodies.